centrifugal concentration

KNF | Lab Applications | Centrifugal Concentration

Centrifugal vacuum concentrators use a combination of centrifugal force, vacuum, and heat to rapidly and gently evaporate multiple small samples to either a dry or a wet pellet state. There are many different applications for centrifugal evaporation, including concentration of polynucleotides and protein samples, residue analysis, concentration ...

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Concentrating Protein in Media Samples with Centrifugal ...

The centrifugal devices used here are available from Pall Corporation and come with a variety of different molecular weight cutoffs (MWCO). We used these devices to concentrate 250 µL of samples containing MMP-2 (50-70 kDA) a total of ten-fold (final volume of 25 µL) for use in gelatin zymography.

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CentriVap® Centrifugal Concentration System Accessories ...

For use with all CentriVap® centrifugal concentration systems, except DNA system.The concentrators assist with the helpful sample preparation for easier molecular biology and organic chemistry analyses. Designed to protect DNA, RNA, protein, and nucleotide temperatures, concentrators reduce the risk of sample dryness that could negatively impact tests.

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Amicon® Ultra 0.5 mL Centrifugal Filters | Life Science ...

Amicon® Ultra-0.5 centrifugal filter devices provide fast ultrafiltration, with the capability for high concentration factors and easy concentrate recovery from dilute and complex sample matrices. The vertical design and available membrane surface area provide fast sample processing, high sample recovery (typically greater than 90% of dilute ...

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Centrifugal freeze concentration - ScienceDirect

The high separation efficiency of solutes with centrifugal freeze concentration is a consequence of using an external driving force (pressure difference induced by centrifugation) much higher than that operating in separation by gravitational thawing.

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Ultra-15 How to Use Amicon® Ultra-15 Centrifugal Filter ...

Factors affecting flow rate include sample concentration, starting vol ume, chemical nature of solute, relative centrifugal force, centrifuge rotor angle, membrane type, and temperature. Figures 1 and 2, and Tables 2 and 3 can be used to estimate the time required to achieve a given volume of filtrate or

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miVac Sample Concentrators - SP Scientific

miVac is a modular range of centrifugal vacuum concentrators and freeze driers capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. Choose from the Duo Concentrator for low sample numbers or …

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Centrifugal evaporator - Wikipedia

A centrifugal evaporator is a device used in chemical and biochemical laboratories for the efficient and gentle evaporation of solvents from many samples at the same time, and samples contained in microtitre plates. If only one sample required evaporation then a rotary evaporator is most often used. The most advanced modern centrifugal evaporators not only concentrate many samples at the same ...

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Centrifugal Concentrators | Ultrafiltration Devices| Sartorius

Spin to Win – Versatile Protein Concentration Solutions. With their broad range of volume capacities and MWCOs, Sartorius ultrafiltration centrifugal concentrators offer the optimal solution to any concentration or buffer exchange application. Our patented dead-stop technology allows direct sample recovery of the concentrate and prevents the ...

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Metals | Free Full-Text | Recovery of Rare Earth Oxides ...

Recovery of Rare Earth Oxides from Flotation Concentrates of Bastnaesite Ore by Ultra-Fine Centrifugal Concentration . by Alex Norgren. 1,2,* and . Corby Anderson. 1. 1. Department of Mining Engineering, Kroll Institute for Extractive Metallurgy, Colorado School of Mines, 1500 Illinois St., Hill Hall 337, Golden, CO 80401, USA. 2.

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Gold Recovery with Centrifugal Gravity Concentrator

Press the "run" button. The bowl will start spinning. Feed slurry into the concentrator. The particles must be smaller than 2 mm. After about 30-60 minutes, stop feeding slurry into the concentrator. Reduce the process water pressure. Press the "decrease speed" button until the display shows 5 Hz.

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Comparison of virus concentration methods for the RT-qPCR ...

Also, centrifugal concentration devices, such as those used in this study, may co-concentrate RT-PCR inhibitors. To obtain information on the RT-PCR inhibitors, 50 mL of distilled water was seeded with MHV and the MHV recovery efficiency was compared to that for the MHV seeded wastewater for Methods C and D. Given that the recovery efficiency ...

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Understanding Concentration and Evaporation Technology ...

Centrifugal concentration. Centrifugal concentrators induce solvent boiling under vacuum and thus samples are cold; however, in contrast to freeze dryers, the samples are not frozen, and so the process can be faster than freeze-drying. Care must be taken with centrifugal evaporation of aqueous samples that are prone to freezing.

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SOLID LIQUID SEPARATION: CENTRIFUGATION

CONCENTRATION Hyperfiltration Ultrafiltration High Speed Centrifugation Microfiltration Conventional Filtration E v a p o r a t i o n 0.1 1.0 10 Concentration ( % dry matter) 100 10 1 0.1 0.01 0.001 S i z e (m i c r o n s) d p2∆ρ dp2∆P for filtration. ... Centrifugal decanter SOLIDS CONTENT. Title: No Slide Title

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Centrifugal Filters - Pall Corporation

Pall's centrifugal filter devices can replace traditional separation techniques, such as column chromatography, preparative electrophoresis, alcohol or salt precipitation, dialysis, and gradient centrifugation, when performing the following: Protein or nucleic acid concentration Desalting Buffer exchange Deproteination of biological samples Fractionation of protein mixtures Separation of ...

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Amicon® Ultra-15 Centrifugal Filter Units - Sample Prep ...

Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E. J Proteome Res. 2006 Apr;5(4):983-7. 2006: Protein Concentration: Ultrafiltration for proteomic sample preparation

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Dandong Gravity Mining Technology Co.,Ltd_Full automatic ...

GTD80 Full automatic centrifugal concentration machine Improvements and updates Benchmark international advanced products After years of experience precipitation and experimental research, our company has upgraded the out-dated centrifugal machine that have been produced for many years!

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Centrifugal Filter Devices | Fisher Scientific

Centrifugal Filter Devices. MilliporeSigma™ Amicon™ Ultra-0.5 Centrifugal Filter Units. Achieve both high sample recovery and fast sample processing. EMD Amicon™ Ultra Centrifugal Filter Units feature regenerated cellulose membranes that are ideal for protein & nucleic acid purification, concentration …

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Alfa Laval - Use of centrifuge - how separation works ...

A disc stack separator separates solids and one or two liquid phases from one another using extremely high centrifugal forces. The centripetal force generated by the rotating bowl pushes solids against the periphery of the bowl, while the less dense liquid phases form concentric inner layers. The separation process can be either a discontinuous ...

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User Guide - Harvard University

Concentration is achieved by ultrafiltering the sample solution through an anisotropic membrane. Centrifugal force drives solvents and low molecular weight solutes through the membrane into the filtrate vial. Retained macrosolutes are above the membrane inside the sample reservoir. As the sample volume is diminished, retained

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Microsep™ Centrifugal Filters (0.5 to 5 ML Sample Volume)

Microsep™ Centrifugal Filters. Precise, Quick Recovery of Microliter Volumes. Rapidly concentrates 0.5-5 mL sample volumes to 65–100 μL. Achieve 50X concentration and > 90% recovery in just minutes. Deadstop feature prevents samples from spinning to dryness.

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Does vacuum centrifugal concentration reduce yield or ...

Vacuum centrifugal (SpeedVac) concentration is commonly applied to nucleic acids extracted from formalin-fixed paraffin-embedded (FFPE) sections, but with an unknown impact. We concentrated DNA and RNA from FFPE biospecimens using different time/temperature SpeedVac combinations of up to …

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Results for case canister centrifugal concentration ...

Labconco CentriVap Centrifugal Concentration System Accessories, Labconco 7815300 Clear Polypropylene Canister With Stand $819.99 (Save $40.00) $779.99. 0. Labconco CentriVap Centrifugal Concentration System Accessories, Labconco 7455200 Rotors 28 …

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MilliporeSigma Amicon Ultra-2 Centrifugal Filter Units ...

Achieve both high sample recovery and fast sample processing. EMD Amicon™ Ultra Centrifugal Filter Units feature regenerated cellulose membranes that are ideal for protein & nucleic acid purification, concentration and desalting. Available in several diameters and molecular weight limits.

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Centrifugal Concentrator at Thomas Scientific

This Amicon Ultra 0.5 mL centrifugal filter enables the concentration of proteins with a membrane NMWL of 10 kDa. Amicon Ultra centrifugal filters are ideal for protein purification, protein concentration & desalting. Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-10 membrane …

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Concentrating Proteins Using Amicon Centrifugal Filters ...

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Optimal inter-batch normalization method for GC/MS/MS ...

We revealed that centrifugal concentration was a critical factor to cause inter-batch variation. Unexpectedly, neither the QC-based normalization methods nor the IS-based method was able to normalize inter-batch variation, though MI- or TI-based normalization methods were effective in normalizing inter-batch variation.

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Advances in Centrifugal Gravity Concentration - Beyond Low ...

The application and effective operation of centrifugal concentration units for gold recovery relies on some key factors of gold's behaviour within grinding circuits. The most important factor is that properly operating hydrocyclones retain almost all of the gravity recoverable gold (GRG) within the grinding circuit where a fraction of the ...

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Ultra-Fine Centrifugal Concentration of Bastnaesite Ore

Historically, the ability to effectively separate carbonate gangue from bastnaesite via flotation has frequently proven to be challenging without sacing significant rare earth oxide (REO) grade or recovery. However, in light of the fact that the rare earth bearing minerals often exhibit higher specific gravities than the carbonate gangue, the possibility exists that the use of gravity ...

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Concentrating Viruses with Centrifugal Ultrafiltration Devices

Concentrating Viruses with Centrifugal Ultrafiltration Devices Jennifer Brum, Tucson Marine Phage Lab April, 2011 Purpose: This protocol describes how to concentrate viruses in liquid samples using an Amicon or Nanosep centrifugal ultrafiltration device.

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How a Centrifuge Works – Federation Of American Scientists

In the spinning rotor, the slightly heavier UF 6 that contains U-238 will be slightly more compressed along the rim relative to the lighter UF 6 that contains U-235, which would have a relatively greater concentration near the axis. The separation between the two isotopes created by the centrifugal forces is quite small. However, there is a simple way to substantially increase the degree of ...

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Pierce™ Protein Concentrators PES, 10K MWCO, 0.5 mL

Catalog number: 88513. Thermo Scientific Pierce Protein Concentrators PES are disposable ultrafiltration centrifugal devices with a polyethersulfone (PES) membrane for concentration, desalting and buffer exchange of biological samples. This product has a molecular-weight cutoff of 10K, and can be used for processing sample volumes up to 0.5 mL.

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High Precision, Advanced centrifugal concentration ...

The centrifugal concentration machines up for sale are from the leading sellers and trusted manufacturers who assure outstanding quality and steady performance for a long time. These motor-operated centrifugal concentration machines are available in various distinct models and their capacities may vary for each.

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